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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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all 21 experiments
500 nt
mRNA_12: 8380 - K12757 MYL12 myosin regulatory light chain 12, at 32509 to 33351
8380
mRNA_13: 8381 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 33714 to 35045
8381
mRNA_14: 8382 - K07199 PRKAB 5'-AMP-activated protein kinase, regulatory beta subunit, at 36682 to 38243
8382
mRNA_15: 8383 - K02321 POLA2 DNA polymerase alpha subunit B, at 39313 to 41292
8383
mRNA_16: 8384 - K03407 cheA two-component system, chemotaxis family, sensor kinase CheA, at 41535 to 45882
8384
Group
Condition
mRNA_12
mRNA_13
mRNA_14
mRNA_15
mRNA_16
8380
8381
8382
8383
8384
growth on ricinoleic acid
YNB Ricinoleic Acid
0.3
-1.8
-2.3
0.1
growth on oleic acid
YNB Oleic Acid
-0.1
-0.6
-2.1
-0.0
facs on lipid accumulation
Sorted High BODIPY Gate
-0.1
-0.3
-1.3
0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
0.3
-0.9
-0.9
-0.0
ypd buoyancy
40 hrs No Buoyancy Separation
-0.6
-0.4
-0.1
0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
0.3
-0.0
-1.2
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
0.3
-0.3
-1.0
0.1
growth on oleic acid
YNB Glucose
0.1
0.1
-1.0
-0.0
ypd buoyancy
40 hrs Low Buoyancy
-0.3
0.1
-0.6
0.0
ypd buoyancy
40 hrs High Buoyancy
-0.3
0.1
-0.5
0.1
growth on ypd
YPD
-0.1
-0.0
-0.5
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
0.1
0.2
-0.7
0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
0.4
0.0
-0.9
0.2
growth on ricinoleic acid
YNB Glucose
0.4
-0.5
-0.0
-0.1
ypd buoyancy
40 hrs Median Buoyancy
0.0
0.0
-0.4
0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
0.2
0.4
-0.8
0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
0.2
0.5
-0.7
0.1
auxotrophy
YNB Glucose 75mM Arginine
0.3
-0.4
0.5
0.1
auxotrophy
Dropout Complete
0.4
0.0
0.0
0.2
auxotrophy
YNB Glucose 75mM Methionine
0.4
-0.2
0.3
0.2
auxotrophy
YNB Glucose
0.3
-0.3
0.5
0.3
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