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Protein
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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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all 21 experiments
500 nt
mRNA_6661: 15029 - K11699 RDR, RDRP RNA-dependent RNA polymerase, at 1375555 to 1377901
15029
mRNA_6662: 15030 - HMMPfam-G-patch domain-PF01585,HMMPfam-RNA recognition motif (a.k.a. RRM, RBD, or RNP domain)-PF14259,ProSiteProfiles-Eukaryotic RNA Recognition Motif (RRM) profile.-PS50102,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157,ProSiteProfiles-G-patch domain profile.-PS50174,SMART-RNA recognition motif-SM00360,SMART-glycine rich nucleic binding domain-SM00443,SUPERFAMILY--SSF54928, at 1379343 to 1382272
15030
mRNA_6663: 15031 - Hypothetical Protein, at 1382729 to 1383844
15031
mRNA_6664: 15032 - K03165 TOP3 DNA topoisomerase III, at 1384031 to 1388134
15032
mRNA_6665: 15033 - K01952 purL, PFAS phosphoribosylformylglycinamidine synthase, at 1389385 to 1393556
15033
Group
Condition
mRNA_6661
mRNA_6662
mRNA_6663
mRNA_6664
mRNA_6665
15029
15030
15031
15032
15033
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.1
-2.3
0.1
-0.2
0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
0.0
-1.7
-0.3
-0.2
-0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
-1.3
0.1
-0.3
-0.5
facs on lipid accumulation
Sorted No BODIPY Gate
0.0
-0.7
-0.4
0.2
-1.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
0.2
-0.5
-0.2
-0.3
-0.7
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
-0.2
-0.2
-0.2
-0.5
growth on ricinoleic acid
YNB Glucose
0.1
-0.7
0.1
-1.0
0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
-0.2
0.3
-0.5
-0.6
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.1
-0.5
-0.0
0.2
-0.2
auxotrophy
YNB Glucose
0.4
-0.3
0.0
-1.1
0.3
facs on lipid accumulation
Sorted High BODIPY Gate
0.2
-1.3
-0.4
1.0
0.2
auxotrophy
YNB Glucose 75mM Methionine
0.1
-0.5
-0.1
-0.3
0.6
growth on ypd
YPD
-0.1
-0.2
0.4
-0.5
0.3
growth on oleic acid
YNB Oleic Acid
-0.0
0.3
0.1
0.2
-0.5
growth on oleic acid
YNB Glucose
-0.0
0.1
0.3
-0.1
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
0.2
0.2
0.0
-0.1
0.1
auxotrophy
YNB Glucose 75mM Arginine
0.2
-0.5
0.2
0.2
0.5
ypd buoyancy
40 hrs Median Buoyancy
-0.0
-0.5
0.3
0.9
0.0
ypd buoyancy
40 hrs High Buoyancy
-0.1
-0.3
0.4
0.0
0.8
auxotrophy
Dropout Complete
0.3
-0.3
0.3
-0.2
0.8
ypd buoyancy
40 hrs Low Buoyancy
0.0
0.5
0.6
0.8
0.3
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