Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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Top 21 experiments (either direction), sorted by average fitness

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500 nt mRNA_4194: 12562 - K01934 MTHFS 5-formyltetrahydrofolate cyclo-ligase, at 1584696 to 1585623 12562 mRNA_4195: 12563 - HMMPfam-Protein of unknown function (DUF2962)-PF11176, at 1585796 to 1586681 12563 mRNA_4196: 12564 - K12343 SRD5A1 3-oxo-5-alpha-steroid 4-dehydrogenase 1, at 1586838 to 1587953 12564 mRNA_4197: 12565 - K00512 CYP17A steroid 17alpha-monooxygenase / 17alpha-hydroxyprogesterone deacetylase, at 1588332 to 1590437 12565 mRNA_4198: 12566 - K00166 BCKDHA, bkdA1 2-oxoisovalerate dehydrogenase E1 component alpha subunit, at 1590487 to 1592266 12566
Group Condition mRNA_4194 mRNA_4195 mRNA_4196 mRNA_4197 mRNA_4198
12562
12563
12564
12565
12566
facs on lipid accumulation Sorted High BODIPY Gate -0.6   -0.4 -0.7 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.6   -0.5 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation 0.1   -0.2 -0.7 -0.3
facs on lipid accumulation Sorted No BODIPY Gate 0.2   -0.3 -0.4 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.3   -0.4 -0.0 0.0
growth on oleic acid YNB Glucose -0.2   0.0 -0.3 -0.3
auxotrophy YNB Glucose -0.3   -0.3 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.6   -0.3 0.4 -0.1
auxotrophy Dropout Complete -0.1   -0.0 0.1 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2   -0.2 -0.3 0.2
growth on oleic acid YNB Oleic Acid -0.2   0.6 -0.2 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -1.1   -0.7 0.8 0.7
growth on ricinoleic acid YNB Glucose -0.0   0.1 -0.3 0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy 0.3   -0.1 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy 0.1   0.2 -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid 0.0   -0.2 0.1 0.4
ypd buoyancy 40 hrs Median Buoyancy -0.5   -0.2 0.8 0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.2   0.1 0.1 0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy 0.5   -0.2 -0.2 0.5
ypd buoyancy 40 hrs High Buoyancy -0.1   -0.2 0.6 0.4
growth on ypd YPD 0.5   0.2 -0.1 0.4
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