Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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Top 21 experiments (either direction), sorted by average fitness

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500 nt mRNA_2020: 10388 - KOG1703 Adaptor protein Enigma and related PDZ-LIM proteins, at 762718 to 765022 10388 mRNA_2021: 10389 - BLAST deacetylase-like protein [Drechmeria coniospora], at 765421 to 766356 10389 mRNA_2022: 10390 - BLAST KLLA0F09339p [Kluyveromyces lactis], at 766628 to 767371 10390 mRNA_2023: 10391 - K04718 SPHK sphingosine kinase, at 767575 to 770006 10391 mRNA_2024: 10392 - K15979 SND1 staphylococcal nuclease domain-containing protein 1, at 770180 to 773514 10392
Group Condition mRNA_2020 mRNA_2021 mRNA_2022 mRNA_2023 mRNA_2024
10388
10389
10390
10391
10392
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -1.7 -0.4 -0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine 0.3 -2.0 0.0 -0.0 -0.2
auxotrophy Dropout Complete 0.2 -1.0 -0.3 -0.3 -0.2
auxotrophy YNB Glucose 0.4 -1.2 -0.1 -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine 0.5 -1.2 -0.2 0.1 -0.4
growth on oleic acid YNB Glucose 0.1 -0.4 -0.1 -0.5 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.2 -0.1 -0.4 0.2
facs on lipid accumulation Sorted Low BODIPY Gate 0.0 -0.4 0.0 0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation 0.3 -0.9 -0.2 0.5 -0.1
growth on oleic acid YNB Oleic Acid 0.1 0.4 -0.3 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.1 -0.1 0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy 0.1 -0.3 0.1 0.1 0.0
facs on lipid accumulation Sorted No BODIPY Gate 0.2 -0.6 0.1 0.0 0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 0.4 0.1 0.3 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy 0.1 0.0 0.1 0.1 -0.2
growth on ricinoleic acid YNB Glucose -0.1 0.4 0.3 0.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 0.4 0.2 0.5 -0.6
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 0.1 0.2 0.3
ypd buoyancy 40 hrs Low Buoyancy 0.1 0.2 0.2 -0.1 0.2
growth on ypd YPD -0.3 0.7 0.1 0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy 0.1 -0.1 0.2 0.4 0.2
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